![]() These mAbs have well-defined target specificities and efficacies for immunolabeling endogenous target proteins in mammalian brain samples by immunoblot (IB) and immunohistochemistry (IHC) applications 4, 5, 6. Moreover, except for those of rabbit origin, within a given species individual mAbs exist as one of multiple IgG subclasses, and those of different IgG subclasses can be multiplexed and separately detected with subclass-specific secondary Abs 3.Īs a consequence of mAb development efforts that span over 30 years, including at the UC Davis/NIH NeuroMab Facility, we have generated a large collection of cryopreserved hybridoma cells producing mouse mAbs. Combinatorial (i.e., multiplex) labeling and detection can be performed using combinations of Abs from different species followed by their detection with species-specific dye-conjugated secondary Abs. Conventional (i.e., non-recombinant) Abs can be produced in a variety of animal species (e.g., mice, rats, rabbits, goats, etc.) as polyclonal Abs, and from clonal hybridoma cell lines as monoclonal Abs (mAbs) 2. Antibodies also have numerous other uses (as agonists/antagonists of target protein function, to purify/capture their target protein or cells expressing that target, etc.). Antibody labeling can be detected with various imaging modalities, allowing for determination of spatial details of protein expression and localization across a wide range of scales, which in neuroscience research can range from single molecules to nanoscale molecular assemblies to cells to intact brain circuits 1. ![]() Antibodies provide specific and effective labeling of endogenous targets in diverse brain samples including those obtained from human donors 1. Using antibodies (Abs) to detect endogenous target proteins in brain samples is foundational to many aspects of neuroscience research. ![]() The NeuroMabSeq website and database and the corresponding recombinant antibody collection together serve as a public DNA sequence repository of mouse mAb heavy and light chain variable domain sequences and as an open resource for enhancing dissemination and utility of this valuable collection of validated mAbs. This enabled their subsequent engineering into alternate forms with distinct utility, including alternate modes of detection in multiplexed labeling, and as miniaturized single chain variable fragments or scFvs. We enhanced the utility, transparency, and reproducibility of the existing mAb collection by using these sequences to develop recombinant mAbs. The resultant set of sequences was made publicly available as a searchable DNA sequence database () for sharing, analysis and use in downstream applications. To enhance dissemination and increase the utility of this valuable resource, we applied a high-throughput DNA sequencing approach to determine immunoglobulin heavy and light chain variable domain sequences from source hybridoma cells. Over 30 years of research and development efforts including those at the UC Davis/NIH NeuroMab Facility have resulted in the generation of a large collection of mouse mAbs validated for neuroscience research. ![]() For a full list of recipes that can be obtained from Celeste, see the link below.The Neuroscience Monoclonal Antibody Sequencing Initiative (NeuroMabSeq) is a concerted effort to determine and make publicly available hybridoma-derived sequences of monoclonal antibodies (mAbs) valuable to neuroscience research. However, the recipe which you will receive is randomly selected. When you talk to Celeste, who appears to watch the Shooting Star, she'll give you a recipe which uses Star Fragments. The Moon is one of the recipes that can be obtained from Celeste during a Meteor Shower.
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